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Cross-linking strategies to study PRC2 regulation. (a) Structure and photo-cross-linking mechanism of photomethionine. (b) H3(23–34)K27photoMet cross-links to the catalytic subunit EZH2. The diazirine cross-linker is shown as a red triangle, the covalent adduct as a red line. (c) Structure and oxidative cross-linking mechanism of DOPA. (d) H3(35–42) cross-links to SUZ12. The DOPA cross-linker is shown as a red hexagon, the covalent adduct as a red line.
