Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Semisynthesis of H3S10ph. A synthetic peptide is converted into an α-thioester in solution with HBTU and benzyl mercaptan (top). Simultaneously, a recombinant fragment with an N-terminal cysteine residue (in place of Thr32) is prepared by site-specific proteolysis using Factor Xa (middle). Joining of the two fragments by NCL yields full-length H3 site-specifically phosphorylated at Ser10. A T32C mutation remains at the ligation junction (below). pg = protecting group.
