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Strategies to fix ligation scars in histone semisyntheses. (a) Conversion of Cys to Ala by desulfurization with Raney nickel in the semisynthesis of H3K9me3. (b) Alkylation of cysteine with bromoethylamine to produce thialysine in the semisynthesis of H4K16ac. (c) Radical-based desulfurization in the semisynthesis of H2B-S14ph.
