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Streamlined expressed protein ligation to synthesize H2B-K120ac. Intein self-assembly is harnessed for affinity purification in a column-format. α-Thioester intermediates are subsequently captured by washing the column with excess thiols. The isolated H2B α-thioester is condensed with a synthetic peptide containing an N-terminal cysteine and the K120ac modification. The ligation product is subsequently desulfurized to render the process traceless.
