Figure 5.

PTM-selective antibodies as tools in chromatin biochemistry. (a) General outline of the chromatin immunoprecipitation (ChIP) workflow. (b) Production of site-specific acetyllysine antibodies using synthetic peptides. Specifically acetylated peptides are used to immunize rabbits to elicit a collection of antibodies that recognize defined acetylation marks. In this example, antibody selectivity was probed using the synthetic peptide substrates (right). Plus symbols denote a strong recognition, (+) stands for weak binding, whereas minus signs indicate no cross-reactivity. Data taken from ref (60).