Figure 57.

Oligonucleosome arrays. (a) Synthesis of asymmetric dinucleosomes using nonpalindromic DNA overhangs and their application in studying histone-DNA contacts. Cross-links are detected through a gel-shift of the ³²P-labeled DNA. (b) BPTF binds its marks (H3K4me3, blue flag, and H4K16ac, green circle) in a mononucleosomal context (black arrow). (c) H2B-K120ub stimulates Dot1 in cis (black arrow), but not toward methylation of adjacent nucleosomes. (d) Clr4/Suv39-mediated spreading of the heterochromatin-associated H3K9me3 mark (red flag). (e) Rpd3s deacetylation is stimulated by H3K36 methylation (orange flag) in an intra- and internucleosomal fashion. Note that Rpd3S recognizes dinucleosomes more readily than similarly modified mononucleosomes.