Figure 58.

Identification of PTM-binding factors using modified chromatin as bait. (a) Synergies and antagonisms between DNA and histone methylation recognition. (b) Peptide- and chromatin-based probes reveal partially overlapping interactors. Here only a few examples are shown: BPTF and CHD4 are associated with chromatin remodeling, ING1 is a transcriptional regulator, TFIID is a general transcription factor complex, SIN3 is a histone deacetylase, PCAF and CDYL are histone acetyltransferases, HP1 is a “glue” for heterochromatin, UHRF is a recruiter of DNA methyltransferase, and NUP93 is a member of the nuclear pore. (c) Examples of H2B-K120ub-binding complexes. (d) Synthesis of a hydrolase-resistant H2B-K120ub analogue.