Table. Detection of LACV RNA and virus isolations in Aedes japonicus mosquito pools from Virginia and West Virginia, USA* .

Collection date	County, state	Trap type†	Pool size‡	LACV detection method	Ct value	MLE (95% CI)
2005 Aug	Wise, VA	Ovitrap	9	RT-PCR	38.04	8.59 (0.54 –41.00)
2008 Jul	Montgomery, VA	Gravid	22	RT-PCR	37.57	4.51 (0.26–22.00)
2009 Jul	Montgomery, VA	Gravid	3	Isolation, RT-PCR	14.00	0.23 (0.01–1.11)
2009 Jul	Craig, VA	Gravid	50	Isolation, RT-PCR	23.00	1.28 (0.07–6.29)
2013 Jun	Fayette, WV	Multiple adult	36	RT-PCR	37.66	13.41 (5.18–29.14)
2013 Jul	Cabell, WV	Multiple adult	1	RT-PCR	34.72	13.41 (5.18–29.14)
2013 Aug	Fayette, WV	Multiple adult	15	RT-PCR	37.35	13.41 (5.18–29.14)
2013 Aug	Fayette, WV	Multiple adult	2	RT-PCR	34.64	13.41 (5.18–29.14)
2013 Sep	Kanawha, WV	Multiple adult	1	RT-PCR	37.43	13.41 (5.18–29.14)

*C_t, cycle threshold; LACV, La Crosse virus; MLE, maximum-likelihood estimate of the proportion of infected mosquitoes; RT-PCR, reverse transcription PCR.
†All mosquitoes were collected from the field as adults except when ovitraps were used to collect eggs. Adults were reared from the field-collected eggs before testing for arboviruses. Multiple adult traps were gravid traps, carbon dioxide–emitting light traps, and BG Sentinel (Biogents AG, Regensburg, Germany) traps baited with octenol lures.
‡Pool size indicates no. adult mosquitoes tested for LACV.