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| Concentration of paclitaxel (mg/ml) | Amount of chitosan as 1st layer (mg/ml) | Sonication time (hr) | Amount of alginic acid as 2nd layer (mg/ml) | Sonication time (mins) | Particle size (nm) | Zeta potential (mV) |
| 0.5 | 0.1 | 1 | 0.1 | 20 | 887 ± 7 | -30 ± 5 |
| 0.5 | 0.1 | 1 | 0.2 | 20 | 240 ± 6 | -35 ± 8 |
| 0.5 | 0.15 | 1 | 0.2 | 20 | 190 ± 8 | -33 ± 3 |
| 0.5 | 0.2 | 1 | 0.3 | 20 | 260 ± 9 | -30 ± 7 |
