Table 1.
Primer sequences and conditions for qualitative real-time polymerase chain reaction.
| Gene | Primer nucleotide sequence | Product size (bp) |
|---|---|---|
| Col-2 | 5′-ATGACAATCTGGCTCCCAACACTGC-3′ (forward) | 364 |
| 5′-GACCGGCCCTATGTCCACACCGAAT-3′ (reverse) | ||
|
| ||
| Sox-9 | 5′-AGCTCACCAGACCCTGAGAA-3′ (forward) | 200 |
| 5′-TCCCAGCAATCGTTACCTTC-3′ (reverse) | ||
|
| ||
| AGC | 5′-CTTGGGCAGAAGAAAGATCG-3′ (forward) | 158 |
| 5′-GTGCTTGTAGGTGTTGGGGT-3′ (reverse) | ||
|
| ||
| OCN | 5′-GGTGCAAAGCCCAGCGACTCT-3′ (forward) | 199 |
| 5′-GGAAGCCAATGTGGTCCGCTA-3′ (reverse) | ||
|
| ||
| Col-1 | 5′-cgttgggaccatcatcacc-3′ (forward) | 102 |
| 5′-cacccatggacattggaggg-3′ (reverse) | ||
|
| ||
| GAPDH | 5′-CTGCCGCCTGGAGAAAG-3′ (forward) | 243 |
| 5′-CGACCTGGTCCTCGGTGTA-3′ (reverse) | ||