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. 2015 Jan 24;66(7):1845–1850. doi: 10.1093/jxb/eru520

Table 1.

Phytozome accession number, its tentative annotation, primer efficiency, forward and reverse primers, and Tm of the primer sequences used in the study

Gene Tentative annotation Primer efficiency Forward sequence Reverse sequence Tm (°C)
Glyma01g06030.1 Expansin 1.85 CTCAATTCCGCTAGAGAGGC CATACACTAACACTTAAATTGAGG 52
Glyma20g04490.1 Expansin 1.89 TGTTGTCCAAAGTTCTAAGTCAC CTAGTATACTAACCAGTCATCA 52
Glyma11g26240.1 Expansin 1.86 ACATTATTGCAAATTTACAATGGG GAATCAATACCTATTAATGACAC 52
Glyma13g24720.1 Extensin 2.00 CGCAGTTCAAGGAGTTGTTTATG TCACAACGGCACCAAGTAG 52
Ef-1 2.00 CTG TAA CAA GAT GGA TGC CAC TAC CAG TCA AGG TTT GTG GAC CT 55.5
UKN 2 1.90 GCC TCT GGA TAC CTG CTC AAG ACC TCC TCC TCA AAC TCC TCT G 57.4