Table 1.
Gene | Tentative annotation | Primer efficiency | Forward sequence | Reverse sequence | Tm (°C) |
---|---|---|---|---|---|
Glyma01g06030.1 | Expansin | 1.85 | CTCAATTCCGCTAGAGAGGC | CATACACTAACACTTAAATTGAGG | 52 |
Glyma20g04490.1 | Expansin | 1.89 | TGTTGTCCAAAGTTCTAAGTCAC | CTAGTATACTAACCAGTCATCA | 52 |
Glyma11g26240.1 | Expansin | 1.86 | ACATTATTGCAAATTTACAATGGG | GAATCAATACCTATTAATGACAC | 52 |
Glyma13g24720.1 | Extensin | 2.00 | CGCAGTTCAAGGAGTTGTTTATG | TCACAACGGCACCAAGTAG | 52 |
Ef-1 | 2.00 | CTG TAA CAA GAT GGA TGC CAC TAC | CAG TCA AGG TTT GTG GAC CT | 55.5 | |
UKN 2 | 1.90 | GCC TCT GGA TAC CTG CTC AAG | ACC TCC TCC TCA AAC TCC TCT G | 57.4 |