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pH dependence and reversibility of cytokinin–receptor interaction. Binding of trans-zeatin to AHK3 (A), AHK4 (B), and ZmHK1 (C) in the plant assay system at different pH values. Reversibility of ligand binding to AHK3 following a pH shift is shown in (D); initial and final pH values are indicated by numbers below the abscissa. TB, NS, and SB indicate total, non-specific, and specific binding, respectively.
