Table 3.
Percent of embryos exhibiting lesions with MTBE, TBA or Formaldehyde exposure
| Control | 0.625mM MTBE |
1.25mM MTBE |
2.5mM MTBE |
5.0mM MTBE |
10.0mM MTBE |
10.0mM TBA |
10.0mM CH2O |
10.0mM TBA+CH2O |
|
|---|---|---|---|---|---|---|---|---|---|
| All Lesions | 15 (± 10) | 40 (± 13) | 45 (± 8) | 45 (± 12) | 75 (± 22)* | 95 (± 9)* | 10 (± 3) | 25 (± 15) | 20 (± 4) |
| Death (by 120hpf) | 5 (± 6) | 5 (± 3) | 5 (± 8) | 5 (± 3) | 5 (± 3) | 5 (± 6) | 5 (± 6) | 5 (± 3) | 5 (± 4) |
| Hatch (at 120hpf) | 80 (± 3) | 75 (± 26) | 60 (± 28) | 60 (± 20) | 65 (± 24) | 45 (± 6)* | 60 (± 13) | 55 (± 9) | 65 (± 11) |
| 3dph Survival | 90 (± 5) | 85 (± 3) | 85 (± 3) | 85 (± 0) | 85 (± 9) | 90 (± 5) | 70 (± 13) | 80 (± 14) | 85 (± 4) |
| Reduced Circulating RBCs | 5 (± 10) | 0 (± 0) | 5 (± 5) | 15 (± 12.5) | 45 (± 30)* | 60 (± 37.5)* | 5 (± 3) | 0 (± 0) | 5 (± 4) |
| Pericardial Edema | 5 (± 5) | 5 (± 5) | 5 (± 5) | 5 (± 5) | 15 (± 10) | 35 (± 15)* | 5 (± 8) | 0 (± 0) | 5 (± 7) |
| Pooled Blood in CCV | 10 (± 10) | 15 (± 5) | 15 (± 5) | 20 (± 15) | 50 (± 5)* | 75 (± 20)* | 0 (± 0) | 5 (± 5) | 5 (± 0) |
| Cranial Hemorrhage | 5 (± 5) | 10 (± 5) | 15 (± 15) | 25 (± 5) | 30 (± 5) | 45 (± 5)* | 5 (± 9) | 10 (± 8) | 5 (± 0) |
| Abnormal ISVs | 5 (± 5) | 15 (± 10) | 15 (± 10) | 20 (± 10) | 35 (± 5)* | 60 (± 5)* | 5 (± 3) | 10 (± 13) | 5 (± 7) |
Values in the table are percentages ± standard deviation based on the average number of embryos in each category from replicate studies. (N=20)
*
Significantly different from control (P ≤ 0.05).