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. Author manuscript; available in PMC: 2015 Mar 30.
Published in final edited form as: J Bone Miner Metab. 2011 Nov 23;30(4):408–418. doi: 10.1007/s00774-011-0336-y

Fig. 3.

Fig. 3

Western blot for sCSF1 using media conditioned by osteoblasts from either wt or k/o mice. Media conditioned for 24 h by MC3T3E1 cells (left lane), primary osteoblasts prepared from neonatal k/o mice (middle lane) or from wt littermates (right lane) was concentrated, desalted, and analyzed by Western blot using a polyclonal antibody to CSF1