Figure 1.

Effect of chemotherapeutic drugs on pancreatic cancer cells. A: Proapoptotic effects of gemcitabine on pancreatic cancer cells. 1.0×10⁶ of pancreatic cancer cells were seeded in culture plates, and incubated with and without 10 μM gemcitabine. After 72 hours, apoptotic cells were measured by FACS analysis. Data shown are mean ± SEM from three experiments. B&C: Effects of various chemotherapeutic drugs on pancreatic cancer cell viability. Cells were treated with increasing concentrations of gemcitabine, 5-FU, and cisplatin (1–100,000 nM). After 72 hours, viable cells were quantified using the MTS reagent. Data shown are mean ± SEM from three experiments. D: log₁₀ (GI₅₀) values and cluster analysis of using log₁₀ (GI₅₀) values were plotted. If log₁₀ (GI₅₀) of the cell lines were below the mean of log₁₀ (GI₅₀) across the panel of cells, the cell lines were defined as sensitive, whereas resistant cell lines had log₁₀ (GI₅₀) values above the mean.