Fig. 2.

a Stages of MYXV-Red morphogenesis in infected B16.SIY cells. Cells were analyzed by TEM 24 h post-inoculation at MOI 10. Immature viral crescents (CR) form in cytoplasmic virus factories (VF). Crescents grow into immature virions (IV) with nucleoid (Nu) that condense to form mature virus (MV). b Multi-step growth kinetics of MYXV-Red in RK13, B16-F10, and B16.SIY cells. HPI = hrs post-inoculation at MOI 0.1. Error bars show mean ± SEM. Data are averaged from three independent experiments. c In vitro B16.SIY cell viability following infection with MYXV. Cells were mock-infected or infected at MOI 1 or 10, and metabolic activity was assessed over 72 h p.i. Viable cells are fluorescent. Data are representative of two independent experiments. Error bars show mean ± SEM, n = 3. *P < 0.05. d In vitro cytopathic effect of MYXV-Red infection (MOI 1) on confluent B16.SIY cells 48 h p.i. Panels one and three were imaged using phase; two and four used a Texas red filter