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. Author manuscript; available in PMC: 2015 Mar 30.
Published in final edited form as: Neuroscience. 2013 Oct 22;256:322–333. doi: 10.1016/j.neuroscience.2013.10.027

Fig. 1.

Fig. 1

Dose response studies in VSC4.1 motoneurons. (A) Determination of residual cell viability following exposure to calcium ionophore (CI) for 24 h. Untreated cells were used as control (CTL). Cells were exposed to 25, 50, 100, 200, and 500 nM CI for 24 h and changes in cell viability were measured by the MTT assay. All experiments were conducted in triplicates and the results were analyzed for statistical significance. Difference in values between CTL and an exposure to CI was considered significant at *p<0.05 or ** p<0.01. (B) Determination of residual cell viability after treatment with different doses (0 to 175 nM) of PPT, WAY, or EST in the CI insulted VSC4.1 motoneurons. Cytoxic insult was induced with exposure to 200 nM CI for 24 h. Then, cells were treated with PPT, WAY, or EST for another 24 h and changes in cell viability were measured by the MTT assay. All experiments were conducted in triplicates.