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. Author manuscript; available in PMC: 2015 Mar 30.
Published in final edited form as: Neuroscience. 2013 Oct 22;256:322–333. doi: 10.1016/j.neuroscience.2013.10.027

Fig. 3.

Fig. 3

Qualitative RT-PCR and real-time qRT-PCR to determine levels of specific miRs in VSC4.1 motoneurons. Treatment groups (24 h): control (CTL), 50 nM PPT, 100 nM WAY,150 nM EST, 200 nM CI, 200 nM CI + 50 nM PPT, 200 nM CI +100 nM WAY, 200 nM CI + 150 nM EST. (A) Qualitative RT-PCR products of miRs were resolved by 2.2% agarose gel electrophoresis. (B) Real-time qRT-PCR products were analyzed for levels of expression of miRs after normalizing with U6 RNA. Difference in values between CTL and a treatment was considered significant at *p<0.05 or ** p<0.01. Difference in values between the CI treated cells and the CI + ER agonist treated cells was considered significant at #p<0.05 or ##p <0.01.