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. Author manuscript; available in PMC: 2015 Mar 30.
Published in final edited form as: Neuroscience. 2013 Oct 22;256:322–333. doi: 10.1016/j.neuroscience.2013.10.027

Fig. 4.

Fig. 4

Overexpression of miR-206, miR-17, or miR-7-1 enhanced the efficacy of WAY or EST for prevention of apoptosis in the CI insulted VSC4.1 motoneurons. Treatment groups: 50 nM miR mimic for 12 h, 50 nM anti-miR mimic for 12 h, 200 nM CI for 24 h, 50 nM miR mimic for 12 h + 200 nM CI for 24 h, 200 nM CI for 24 h + 100 nM WAY for 24 h, 50 nM miR mimic for 12 h + 200 nM CI for 24 h + 100 nM WAY for 24 h, 200 nM CI for 24 h + 150 nM EST for 24 h, and 50 nM miR mimic for 12 h + 200 nM CI for 24 h + 150 nM EST for 24 h. Cells were collected for Annexin V-FITC/PI double staining and flow cytometry to determine the percentages of apoptotic cells from three independent experiments for miR-206 set (A), miR-17 set (B), and miR-7-1 set (C). Difference in values between CI insulted cells and miR mimic + CI, CI + ER agonist, or miR mimic + CI + ER agonist treated cells was considered significant at #p<0.05 or ##p <0.01.