Table 1.

Primers for determining levels of mRNA of ERα, ERβ, Bax and Bcl-2 and also expression of specific miRs in VSC4.1 motoneurons

Primers for target genes	Sequences
ERβ	Forward: 5′-TCC CTC TTT GCG TTT GGA CTA-3′ Reverse: 5′-TTC CCG GCA GCA CCA GTA ACC-3′
ERα	Forward: 5′-AAT TCT GAC AAT CGA CGC CAG-3′ Reverse: 5′-GTG CTT CAA CAT TCT CCC TCC TC-3′
Bax	Forward: 5′-GCA GAG AGG ATG GCT GGG GAG A-3′ Reverse: 5′-TCC AGA CAA GCA GCC GCT CAC G-3′
Bcl-2	Forward: 5′-GGA TGA CTT CTC TCG TCG CTA C-3′ Reverse:5′-TGC AGA TGC CGG TTC AG-3′
GAPDH (control for mRNA)	Forward: 5′-TTC ACC ACC ATG GAG AAG GC-3′ Reverse: 5′-GGC ATG GAC TGT GGT CAT GA-3′
miR-206	Forward: 5′-ACA TGC TTC TTT ATA TCC CCA-3′ Reverse: 5′-AAA CCA CAC ACT TCC TTA CAT TC-3′
miR-17	Forward: 5′-GCA GGA AAA AAG AGA ACA TCA CC-3′ Reverse: 5′-TGG CTT CCC GAG GCAG-3′
miR-21	Forward: 5′-GCT TAT CAG ACT GAT GTT GAC TG-3′ Reverse: 5′-CAG CCC ATC GAC TGG TG-3′
miR-106b	Forward: 5′-TAA AGT GCT GAC AGT GCA GAT AGT G-3′ Reverse: 5′-CAA GTA CCC ACA GTG CGG T-3′
miR-7-1	Forward: 5′-TGG AAG ACT AGT GAT TTT GTT GT-3′ Reverse: 5′-AGA CTG TGA TTT GTT GTC GAT T-3′
U6 RNA (control for miR)	Forward: 5′-CTC GCT TCG GCA GCA CA-3′ Reverse: 5′-AAC GCT TCA CGA ATT TGC GT-3′