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. Author manuscript; available in PMC: 2015 Mar 30.
Published in final edited form as: J Cell Biochem. 2011 Feb;112(2):463–475. doi: 10.1002/jcb.22934

Fig. 4.

Fig. 4

Effects of STAT3 inhibition on melanoma cell apoptosis. A: Dose-dependent effect of STAT3 inhibitor-6 (S3I-201) on STAT-dependent luciferase activity. WM9 cells were transiently transfected with 3xSTAT-Luc reporter and then treated for 6 h using S3I-201. Western blot analysis of phospho-Tyr705-STAT3 and COX-2 levels after 6-h exposure with S3I-201. Actin was used as a loading control. B: Surface expression levels of DR5 were determined for indicated cell lines using with anti-DR5-PE mAb and flow cytometry. C: Effects of S3I-201 (50 µM) alone or in combination with TRAIL on cell cycle-apoptosis in LU1205 and WM9 melanoma cells. Results of a typical experiment are presented (one from three). D: Clonogenic survival assay of LU1205 and WM9 cells 12 days after treatment with S3I-201 (50 µM), TRAIL (30 ng/ml) or their combination. Double star indicates zero survival of WM9 cells after combined treatment. Error bars represent mean ± SD (Student's t-test, P < 0.05).