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. Author manuscript; available in PMC: 2015 Mar 30.
Published in final edited form as: J Cell Biochem. 2011 Feb;112(2):463–475. doi: 10.1002/jcb.22934

Fig. 6.

Fig. 6

Effects of downregulation of COX-2 on TRAIL-induced apoptosis. A: Effects of NS398 (50 µM), an enzymatic inhibitor of COX-2, on TRAIL (30ng/ml) or TRAIL+ CHX (1 µg/ml) induced apoptosis in fibroblasts and indicated melanoma lines. B: Effects of COX-2 suppression by specific COX-2 RNAi on COX-2 and cFLIP-L protein expression levels in WM9 and LU1205 melanoma cells. Cell cultures stably transfected by control or COX-2 RNAi were previously described. C: TRAIL-induced apoptosis in control and COX-2 deficient cell cultures. Apoptotic levels were determined 48 h after treatment using PI staining and the flow cytometry. Error bars represent mean ± SD (Student's t test, P <0.05).