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. Author manuscript; available in PMC: 2016 Mar 9.
Published in final edited form as: Curr Protoc Bioinformatics. 2015 Mar 9;49:8.19.1–8.19.16. doi: 10.1002/0471250953.bi0819s49

Figure 4.

Figure 4

A) A 3d scatterplot illustrating three different areas in the three-dimensional MiST feature space: (1) biologically relevant interactions (red gradient, MiST scores > 0.75) are specific and reproducible with variable abundance (2) non-specific, reproducible interactions are often background proteins and (3) specific, irreproducible interactions are often one-hit-wonders including contaminants and MS artifacts B) The scored bait-prey list at a high MiST score threshold visualized as a protein interaction network with Cytoscape. Red edges depict interactions identified by AP-MS while black edges are mined from the CORUM database. Edge width corresponds to the MiST score. High MiST scores for multiple subunits of a described complex add confidence to observed AP-MS interactions.