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BCB staining. (A) Oocytes observed under phase-contrast microscope (magnification, 100×) after incubation with 26 μM BCB stain. Dark and light arrows indicate BCB− and BCB+ oocytes, respectively. BCB− (B) and BCB+ (C) oocytes at 200× magnification. Scale bar, 100 μm. Blastocysts from IVF (D), BCB+ (E), and BCB− (F) oocytes.
