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. 2015 Mar 30;10(3):e0124063. doi: 10.1371/journal.pone.0124063

Fig 2. swi7H4 mutant cells exhibit chromosome segregation defect at non permissive temperature.

Fig 2

(A) Cells were grown at 25°C till mid log phase and then shifted at 36°C for 6 hr, fixed with 70% ethanol and stained with DAPI to visualize nuclei (upper panel) or with differential interference contrast (DIC) filter (lower panel). Arrow indicates defective chromosome segregation. (B) Indicated strains were grown till mid log phase at 25°C and then shifted at 36°C. Cells were collected at indicated time interval, fixed with 70% ethanol and stained with DAPI. Cells having aberrant chromosome segregation were counted.