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. 2015 Mar 30;10(3):e0124063. doi: 10.1371/journal.pone.0124063

Fig 4. swi7H4 mutant cells delay the mitotic entry and activates checkpoint kinase Chk1.

Fig 4

(A) Cells were processed as in Fig 3, samples were collected at 15 minute interval and the number of cells having two nuclei was counted using DAPI as an indication of cells entering into mitosis. (B) Indicated strains were grown at permissive temperature till mid log phase then shifted at 36°C for indicated time. Protein lysate was prepared as described in Material and Methods, samples were run on 8% SDS PAGE, transferred on nitrocellulose membrane and probed with anti HA antibody.