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. 2015 Mar 30;10(3):e0121056. doi: 10.1371/journal.pone.0121056

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© 2015 Forner et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — The variant 34 amino acid (aa) repeats each binding a defined nucleotide as specified by their repeat-variable diresidues are depicted as cyan and magenta boxes. The invariant first and last repeats are marked in green. The part originating from the original TALEs is marked by an orange line. This part and the FokI domain are drawn to scale. For the first generation TALENs, one amino acid (alanine) has been inserted between aa1 and aa2 to create an NcoI site. The S-tag versions are 6 aa longer. (B) Binding sites of the TALENs used in this study. (C) Constructs for TALEN expression. pJF802 & pJF803 are built similarly to pJF752 & pJF753 but contain the P16 promoter instead of the CLV3 promoter and the RBCS terminator instead of the CLV3 terminator. LB: left border. RB: right border. p: promoter. t: terminator. attB1/2: attachment sites from Gateway cloning. Basta^R: phosphinotricine acetyl transferase. Kan^R: neomycin phosphotransferase. NOS: nopaline synthase. NLS: nuclear localization signal.