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. 2015 Mar 30;10(3):e0121161. doi: 10.1371/journal.pone.0121161

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© 2015 Fausther et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — (A) Immortalized rat PF RGF and RGF-N2 protein samples were analyzed for expression of Gfap and cytokeratins. Housekeeping Gapdh protein was used as loading control, Mz-Cha-1 cell lysate as positive control for cytokeratin expression, and HSC-T6 cell lysate as positive control for Gfap expression. RGF (passage #59, 1^st lane and passage #34, 2^nd lane) and RGF-N2 (passage #52, 1^st lane and passage #25, 2^nd lane) cells do express neither cytokeratin proteins nor Gfap protein (after 45 passages for the latter). (B) Immortalized rat PF RGF and RGF-N2 protein samples were analyzed for expression of αSMA, Elastin, Pdgfrβ and Egfr. RGF and RGF-N2 express all proteins tested. Housekeeping Gapdh protein was used as loading control. kDa, kiloDaltons.