Fig 4. Plasmid DNA transfer in immortalized rat portal fibroblastic RGF and RGF-N2 cell lines by immunofluorescence.

RGF and RGF-N2 cells were transfected with an expression vector encoding monomeric GFP protein cDNA using Fugene6 transfection reagent. Transfected cells were then fixed and counterstained with DAPI dye, before confocal microscopy imaging. Both cell lines exhibit green fluorescence signal, indicative of recombinant GFP protein expression. 200X magnification.