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. Author manuscript; available in PMC: 2015 Sep 4.
Published in final edited form as: Mol Cell. 2014 Nov 13;56(5):696–707. doi: 10.1016/j.molcel.2014.10.011

Figure 4. Adenylation of miRNAs is globally decreased in wisp mutant.

Figure 4

(A) Proportion of each RNA class in the sequencing libraries from wild type and wisp mutant eggs (0–2 h AEL). (B) A scatter plot showing the differences of adenylation ratio versus those of miRNA read counts between wild type and wisp mutant eggs. The Spearman correlation coefficient is −0.496. The number of miRNA species (n) in each quadrant is presented. Forty six miRNA species were analyzed (reads per million (RPM) > 50 in at least one of the two samples). (C) MiRNA modification determined by sequencing. Adenylation ratio is significantly reduced in wisp mutant eggs, but other modification ratios were not altered. (D) Length distribution of miRNAs in wild type and wisp mutant eggs. The mean lengths are 22.1 nt in wild type, and 21.7 nt in wisp mutant. (E) Adenylation of 5p miRNAs and 3p strand miRNAs. (F) Examples of sequencing reads mapped to the representative miRNAs in eggs. Reference sequences according to miRBase are highlighted in gray boxes. Nontemplated A addition is indicated in blue letters highlighted in yellow. The RPM values of each sequence are presented on the left side.