Fig 4. Chloro simultaneously inhibits Ca²⁺ sparks and STOCs.

(A, B) A mouse tracheal smooth muscle cell was patched and held at −40 mV. Ca²⁺ sparks and STOCs were simultaneously recorded at 0 mV, which were blocked after adding 1 mM chloro. Ca²⁺ spark-triggered STOCs are indicated by the dashed lines. (C, D) The frequency and amplitude of Ca²⁺ sparks and STOCs from 5 cells are summarized. These data further demonstrate that the disappearance of STOCs will be due to the blockade of RyRs by chloro.