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. 2015 Mar 30;10(3):e0122897. doi: 10.1371/journal.pone.0122897

Fig 2. Maps of Mycobacterium—E. coli shuttle vectors harboring the pMyong2 vector system, and the stability and compatibility of the pMyong2-TOPO vector system.

Fig 2

(A) Maps of the pMyong2-Topo vector, which replicates in mycobacteria and E. coli, (B) pMyong2-EGFPh expressing EGFP under the mycobacterial hsp65 promoter, (C) pMyong2-EGFPe expressing EGFP under the CMV promoter, and (D) pMyong2-hMIF expressing the hMIF gene under the mycobacterial hsp65 promoter. (E) M. smegmatis, carrying the pMyong2-TOPO (black column) or pSE100 (empty column) vectors, was plated onto 7H10 agar medium supplemented with antibiotics. (F) M. smegmatis carrying pMyong2-TOPO and pSE100 vectors was plated onto 7H10 agar medium supplemented with either or both antibiotics (gray column, kanamycin;empty column, hygromycin;black column, kanamycin and hygromycin). (G) M. smegmatis co-transformed with pAL5000-mCherry and pMyong2-EGFP-Hyg vectors was observed using confocal microscopy. Abbreviations: Ampr, ampicillin resistance; Kmr, kanamycin resistance; pUC ori, bacterial origin of replication; oriM, mycobacterial origin of replication; CMV, cytomegalovirus promoter from pIRES2-EGFP vector; EGFP, EGFP gene from pIRES2-EGFP vector; Phsp, hsp65 promoter from M. bovis BCG; Km, kanamycin; Hyg, hygromycin.