Table 3. Phenotypes of cell lines generated using the inducible HSV1-IE bidirectional promoter and Sleeping Beauty transposon-mediated integration.

	GFP Expression for Clonal Cell Populations
Criteria	Leaky	Uninduced	Heterogeneous	Optimal
No Dox (MFI)	101 ± 10	12 ± 2	38 ± 6	14 ± 3
Plus Dox (MFI)	473 ± 21	28 ± 11	177 ± 73	208 ± 56
Fold Induction	5 ± 0.3	3 ± 2	5 ± 1	15 ± 2
% Induced a	20 ± 0.1	32 ± 22	40 ± 1	89 ± 3
# of Clones/Total Clones	2/14	3/14	2/14	7/14
Frequency (%)	14.3	21.4	14.3	50

^apercent of doxycycline induced cells per clone.

MFI, mean fluorescence intensity. Values for No Dox, Plus Dox, and Fold Induction are mean ± s.e.m.