Table 4. Gene expression levels achieved using the inducible, HSV IE bidirectional promoter.
GFP and NGFR MFI for Clonal Cell Populations | ||||
---|---|---|---|---|
Treatment | GFP | Fold-Increase | NGFR | Fold-Increase |
No Dox | 13 ± 4 | —— | 801 ± 95 | —— |
No Dox, Plus VP16 | 33 ± 14 | 2.6 ± 0.42 | 774 ± 30 | 1.0 ± 0.04 |
Plus Dox | 119 ± 15 | 9.1 ± 0.13 | 883 ± 36 | 1.1 ± 0.04 |
Plus Dox, Plus VP16 | 1091 ± 96 | 83.9 ± 0.09 | 792 ± 40 | 1.0 ± 0.05 |
MFI, mean fluorescence intensity. Values are mean ± s.e.m., n = 3 experiments for two independent cell lines.