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. 2015 Mar 30;11(3):e1005109. doi: 10.1371/journal.pgen.1005109

Fig 3. Esp1 physically interacts with Ty1-IN.

Fig 3

(A) Ty1 peptides identified in Esp1-Myc mass spectrometry versus untagged (mock) strain are color coded as follows: Gag/coat protein (blue), PR (yellow), IN (orange) and RT/RNAse H (green). (B) Immunoblot of whole cell lysate (Lysate) and GFP-Trap IP carried out from untagged wild type (No Tag), Esp1-GFP, Scc1-GFP, Pds1-GFP and Ndc80-GFP cells. Expression of a Ty1 element (pGAL1-Ty1-H3) was induced in all strains for 24 hours prior to cell lysis. Blots were probed with anti-GFP and anti-IN (8b11) antibodies. (C) Immunoblot of whole cell lysate (Lysate) and GFP-Trap IP carried out from Esp1-Myc or untagged wild type (No Tag) cells carrying a pGAL-GFP-LacZ-Ty1-IN plasmid (Ty1-IN) or pGAL-GFP-lacZ (vector) control. Cells were either grown in glucose (D) or galactose (GAL) for 24 hours to repress or induce GFP-LacZ-Ty1-IN expression, respectively. The asterisk marks a background band that is present in the lysate of the cells grown in glucose but is not detected in the GFP-Trap IP.