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. 2015 Mar 30;11(3):e1005109. doi: 10.1371/journal.pgen.1005109

Fig 8. Esp1 and Pds1 are required for Ty1 element insertion upstream of the SUF16 locus.

Fig 8

(A) Schematic of the SUF16 genomic locus with PCR primers (arrows) designed to hybridize within the SNR33 gene and the newly inserted Ty1 elements. (B) and (C) PCR analysis of yeast genomic DNA extracted from indicated strains grown in triplicate for 3 days at 20°C to induce transposition. Upper panel is the result of the PCR assay with primers shown in (A) whereas lower panel is a control PCR for the CPR7 locus to demonstrate that yeast genomic DNA was present in each sample. Quantification of Ty1 insertion events is shown relative to wild type (WT) as described in the Materials and Methods.