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. 2015 Mar 30;11(3):e1005109. doi: 10.1371/journal.pgen.1005109

Fig 10. The mitotic exit network has a role in Ty1 transposition.

Fig 10

(A) Transposition frequency of wild type (WT), cdc14-1, cdc5-1, cdc15-2, dbf2-1, spo12Δ and slk19Δ cells carrying a plasmid with a marked Ty1element (pBDG922). Mutants with transposition frequencies that are significantly different from WT are shown with three asterisks (p<0.0005). (B) PCR analysis of yeast genomic DNA extracted from indicated strains grown in triplicate for 3 days at 20°C to induce transposition. Upper panel is a SUF16 PCR assay whereas the lower panel is a control PCR for the CPR7 locus to demonstrate that yeast genomic DNA was present in each sample. Quantification of Ty1 insertion events is shown relative to WT as described in the Materials and Methods.