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. 2015 Mar 30;10(3):e0122060. doi: 10.1371/journal.pone.0122060

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© 2015 Chou et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — (A) Liver sinusoidal endothelial cells were isolated from mouse liver tissue and their purity was determined by CD45 (left panel) and CD31 (right panel) positives rate by flow cytometry analysis. (B) Liver sinusoidal endothelial cell purity during five serial passages. (C) LPA receptors’ mRNA expression determined by qRT-PCR. LPAR1 and LPAR6 mRNA expression was normalized to that of GAPDH mRNA expression (n = 3). (D) Strategy for investigating LPA effects on liver sinusoidal endothelial cells using proteome profile arrays.