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. 2015 Mar 30;10(3):e0122060. doi: 10.1371/journal.pone.0122060

Fig 5. LPA effects on angiogenesis factor, cytokine, and chemokines expression are mediated by LPAR1 and LPAR3 signaling.

Fig 5

(A) LPAR1 and LPAR3 signaling effects on LPA induced proteins level. Liver sinusoidal endothelial cells were pre-treated with an inhibitor of LPAR1 and LPAR3, ki16425 (1 uM), for 30 minutes prior 5 uM LPA treatment. After 24 hours, conditioned media derived from vehicle (1% BSA), LPA (5uM) alone, and ki16425 plus LPA treatment were collected for protein level determinations by EIA. Data shown are fold changes of induction with LPA alone versus vehicle treatment, and ki16425 treatment combined with LPA versus vehicle treatment. Results were compared between LPA treatment alone and ki16425 treatment combined with LPA (n = 3); *p < 0.05. (B) Time course for LPA effects on specific genes’ mRNA expressions. Liver sinusoidal endothelial cells were treated with vehicle (1% BSA) or LPA (5 uM). After 4, 8, and 16 hours, total RNA was isolated from vehicle and LPA treated cells for mRNA determinations by qRT-PCR. Data are fold changes of induction with LPA treatment versus vehicle treatment. Results were compared with vehicle treatment (n = 3); *p < 0.05.