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. 2015 Mar 30;10(3):e0122440. doi: 10.1371/journal.pone.0122440

Fig 1. Apoptotic cells inhibit IL-1β secretion and processing in response to DSS is mediated by the NLRP3 inflammasome.

Fig 1

(A) pMΦ from wild type (WT) and NLRP3-deficient mice (Nlrp3 -/-) and following 4 weeks of cohousing, were treated with 3% DSS in the presence of LPS priming. (B) Influence of apoptotic cell treatment on IL-1β release by pMΦ. Macrophages were treated with apoptotic cells (1:8) prior to LPS and 3% DSS treatment. IL-1β was determined in the supernatant by ELISA. Shown are representative data as means ± SEM of 3-to-5 independent experiments done in triplicate (*p<0.01 t-test). (C) The inhibition effect is a direct consequence of apoptotic cell recognition, independent of engulfment. Macrophages were incubated without or with 2 μM cytochalasin D for 45 min before the addition of apoptotic cells and DSS challenge. Shown are representative data as means ± SEM of 2 independent experiments done in triplicate (*p<0.01, one way ANOVA).