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. 2015 Mar 30;10(3):e0123960. doi: 10.1371/journal.pone.0123960

Fig 6. Immunohistological analysis of polySia+cells and cell proliferation status in early and late postnatal mice epididymis.

Fig 6

Paraffin-embedded serial section of 4 days old (A-C) and 25 days old (D-F) mouse epididymis (cauda). Sections were stained with a mAb against proliferating cell nuclear antigen (anti-PCNA) for proliferating status (A,D) and additional stained with a mAb against polySia (B,E). For negative control, tissue sections were pretreated with endoN to degrade polySia (data not shown). For identification of smooth muscle cells a mAB against smooth muscle actin were used (C,F). * labels same positions of smooth muscle cells. Tissues were counterstained with Haemalaun. Scale bars representing 20 μm.