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Plot shows the enthalpy (kcal/mol of injectants) change obtained through single injection based mixing of 20 μM CaM-free iNOSfl with 8 μM of CaM. Data analysis through one site curve fitting is reported for the binding of CaM to iNOSfl expressed and purified in absence of CaM. Results shown are representative of three independent experiments done under similar conditions.
