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. 2015 Mar 30;10(3):e0117190. doi: 10.1371/journal.pone.0117190

Fig 8. Pre-treatment with DJ-1 derived peptide (ND-13) protected against SIN-1 and glutamate-induced toxicity in vitro.

Fig 8

NSC-34 cells were exposed to peroxynitrite ion generator 3-morpholinosydnonimine (SIN-1), and cell survival was evaluated by Alamar blue assay (A). The experiment was reapeted 3 times and the results are presented as averages ± SD. * p<0.05. Glutamate-induced apoptosis was measured by Annexin V-FITC, PI staining using FACS analysis (B). Different stages of apoptosis were differentiated as follows: early apoptosis (Annexin V+/PI−); late apoptosis/necrosis cells (Annexin V+/PI+); and viable cells (Annexin V−/PI−). Histograms represent the proportion of apoptotic cells relative to total cells. Plots were calculated from the histogram distributions (avarages ± SD). # p<0.05; ## p<0.01; ***, ### p<0.001; * vs. control; and # vs. glutamate-treated group.