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. Author manuscript; available in PMC: 2015 Nov 6.
Published in final edited form as: Cell. 2014 Nov 6;159(4):869–883. doi: 10.1016/j.cell.2014.10.019

Figure 7. Loss of EZH2 from Target Genes as a Result of ATRX Knockdown Results in Activation of These Genes.

Figure 7

(A, B) Left panel: ChIP-seq tracks of the Hoxd cluster (A) or individual genes (B) showing EZH2 and H3K27me3 patterns in ATRX+ and ATRX-KD MEFs. Black bars, statistically significant enriched segments. H3K4me3 (pink) and RNA Seq (blue) tracks were previously published (Yang et al., 2010; Yildirim et al., 2012).

(C) qRT-PCR analysis of expression levels before and after ATRX KD. Averages and SE of three independent experiments shown with Student’s t test P values.

(D) EZH2 and H3K27me3 coverages over TSS before and after ATRX KD.

(E) Model: ATRX-dependent targeting of PRC2 on a genome-wide scale. ATRX mediates targeting either by directly binding DNA, or via regulatory RNAs such as those in the PRC2 interactome.