Fig 3. Matrix-metalloproteinase (MMP) gelatinase activity and reactive oxygen species (ROS) production in the heart tissues of TFAM mice and Twinkle (TW) mice at 8weeks after creating arteriovenous fistula.

(A) Representative images and quantification of MMP gelatinase activities in LV from TFAM or TW mice at 8 weeks after creating AVF (n = 6), **P < 0.01 vs. WT+Sham, ^† P < 0.01 vs. WT+VO, ^†† P < 0.01 vs. WT+VO, analyzed by one-way ANOVA followed by post hoc Tukey’s test. (B) Representative images and quantification of Dihydroethidium staining (DHE) staining of LV from TFAM or TW mice (n = 6), **P < 0.01 vs. WT+Sham, ^† P < 0.01 vs. WT+VO, ^†† P < 0.01 vs. WT+VO, analyzed by one-way ANOVA followed by post hoc Tukey’s test. Scale bar, 100μm. All data are mean±SEM.