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. 2015 Mar 30;10(3):e0119687. doi: 10.1371/journal.pone.0119687

Fig 5. Effect of TFAM overexpression on mitochondrial DNA (mtDNA), mitoROS and mRNA expressions of matrix-metalloproteinase (MMP)-2 and MMP-9 in rat neonatal myocytes.

Fig 5

(A) Human TFAM expression at 72 h in neonatal rat ventricular myocytes infected by LacZ (control)-adenovirus or TFAM-adenovirus (multiplicity of infection [MOI] = 0.3, 1, and 3). (B) mtDNA copy number in TFAM-adenovirus infected myocytes at 72 h after infection (1 MOI) (n = 4–5). (C) Rotenone (Rot) or DMSO (Vehicle [Veh])-induced mitoROS detected by dihydroethidium (DHE) staining in LacZ- or TFAM-adenovirus (1 MOI) infected myocytes. Scale bar, 100 μm (left panel). Quantification of fluorescence intensity in LacZ- or TFAM-adenovirus (1MOI) infected myocytes (right panel; n = 3). (D) Rotenone-induced mRNA expressions of matrix-metalloproteinase (MMP)-2 (left panel) and MMP-9 (right panel) in LacZ- or TFAM-adenovirus (1 MOI) infected myocytes (n = 3), as measured by real-time PCR. Data are expressed as mean ± SEM. *P < 0.05, **P < 0.01, analyzed by one-way ANOVA followed by post hoc Tukey’s test.