Fig 4. MYO3A tail binding MORN4 demonstrated by GST-pulldown assays.

(A) Schematic map of the constructs used for analysis of the MYO3A and MORN4 protein interaction. (B) Protein blots show GST-MORN4 (lane 1) and GST-only (lane 2) proteins were expressed and purified from bacterial lysates. Protein blots confirmed that lysates from transfected COS-7 cells express the GFP-MYO3A tail FL protein (lane 3), and this protein could be pulled out of the lysate with GST-MORN4-glutathione-agarose beads (lane 4) but not with GST- only glutathione-agarose beads (lane 5). Anti-MYO3A tail tip primary antibodies (PB638) were used to detect the GFP-MYO3A tail FL protein in the lysate and pull down fractions.