Fig 5. A) Analysis of CagA expression and CagA tyrosine phosphorylation in the ccrp59 mutant.

Bacterial lysates from H. pylori wild type KE and ccrp59 mutant KE-59PCAT were prepared respectively. Each sample that consisted of equivalent amounts of protein was subjected to immunoblotting assay using antiserum against CagA. AGS cells were not infected or infected with these strains at an MOI of 100 for 4h and subjected to immunoblotting analysis using specific antibody against phosphorylated CagA (Cag-P). B) Control experiment showing that CagA of the ccrp59 mutant can be phosphorylated in vitro by mixing H. pylori lysates with AGS cell lysates.