|
Strains
|
|
|
|
E.coli
|
|
|
| DH5α |
F-, φ80dlacZΔM15, Δ(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rk-, mk+), phoA, supE44, λ-, thi-1, gyrA96, relA1 |
Bethesda Research Laboratories |
|
H. pylori
|
|
|
| G27 |
wt, clinical isolate |
|
| G27ΔCagE |
G27, cagE::cat
|
A. Covacci |
| G27-59CAT |
G27, ccrp59::cat
|
[28] |
| KE88-3887 |
piglet-passaged strain 26695 |
[72] |
| KE-58PCAT |
KE, ccrp58::PCAT
|
[29] |
| KE-59PCAT |
KE, ccrp59::PCAT
|
[28] |
| KE-1142PCAT |
KE, ccrp1142::PCAT
|
[29] |
| KE-1143PNEO |
KE, ccrp1143::PNEO
|
[28] |
| KE- DKO59-1143 |
KE, ccrp59::PCAT, ccrp1143::PNEO
|
[28] |
| KE-VKO |
KE, ccrp58, ccrp59::PCAT, ccrp1142,ccrp1143::PNEO
|
This study |
| KE-59PCAT-K |
KE, ccrp59::PCAT, cagA::Ppfr-ccrp59
|
This study |
| KE-59PCAT-K2 |
KE, ccrp59::PCAT, rdxA::Pccrp59—ccrp59
|
This study |
|
Plasmids
|
|
|
| pTnMax5 |
lacIq, tnpR, tnpA, res, ori
fd, cat
GC, CmR
|
[73] |
| pZERO-2 |
Cloning vector, MCS in lacZ', neo, Kmr
|
Invitrogen |
| pZERO-1 |
Cloning vector, MCS in lacZ', zeo, Zeor
|
Invitrogen |
| p58-59-PCAT |
pZERO-2, Δ HP0058-0059::Pcat, Cmr, Kmr
|
This study |
| p1142-1143-PNEO |
pZERO-1, Δ HP1142-1143::Pneot, Zeor, Kmr
|
This study |
| pSLpPFR59KM |
pSL-Ppfr1021 cm containing ccrp59 and a kanamycin resistance cassette instead of chloramphenicol |
This study |
| pureAB-PCAT |
pZERO-2, Δ ureAB::Pcat, Cmr, Kmr
|
This study |
| pRDX-K-Pccrp59 |
pRDX-C-Pccrp59
ccrp59 containing ccrp59 with its own promoter and a kanamycin resistance cassette instead of chloramphenicol |
This study |
|
Primer
|
|
|
| 0057-L1 |
GCGGTAATTAATTCTCATTC |
This study |
| PCAT-KP 58-59-R1 |
GGCGGATTAACAAAAACCGGAAAGAATATCTCCTTTTATCT |
This study |
| CAT-KP 58-59-L1 |
TGGCAGGGCGGGGCGTAATTAAAGGAGAAATATCATGG |
This study |
| 0060-R3 |
TTAGACAAGCTAGGCACATC |
This study |
| UREB-L1 |
GGGGTTTCTAATGTTTTAGACCGG |
This study |
| CATUREB-R1 |
CTCCTGAAAATCTCGGACTTATTCTCCTATTCTTAAAGTG |
This study |
| CATUREB-L1 |
TGGCAGGGCGGGGCGTAAGATTTTTTAGGAGCAACGCTCC |
This study |
| UreB-R1 |
GCTACGAATAAGCTATACCAAG |
This study |
| HP0059-5Kpn |
gtgcctggtaccTCATAAAATGGGAACATTCATTGAA |
This study |
| HP0059-3Pst |
aaaccactgcagttatggttttggttgttttgaggg |
This study |
| P-Ccrp59-L1 (BamHI) |
GCAGGATCCGGTGGTCTTAATCGGCTATA
|
This study |
| CATS1 |
TCCGGTTTTTGTTAATCCGCC |
This study |
| CATAS1 |
TTACGCCCCGCCCTGCCA |
This study |
| CATS2 |
TCCGAGATTTTCAGGAG |
This study |
| KMS1 |
AAAATTGGAACCGGTACG |
This study |
| KMAS1 |
AGACATCTAAATCTAGG |
This study |
