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. 2015 Mar 30;10(3):e0121720. doi: 10.1371/journal.pone.0121720

Fig 3. Sustained HoxA5 expression inhibits wound healing.

Fig 3

(A) Wound closure in tTA (diamonds) and HoxA5-tTA (circles) mice. Wounds were measured every 4 days. HoxA5-tTA mice showed a significant (P<0.01) impairment in closure at day 4 as compared to tTA mice (n = 8). Mice had received Dox for 3 weeks during gestation (+Dox) and then removed for 5 weeks and during the subsequent wound study (-Dox). (B) Immunofluorescent analysis of vascular density in wound tissue in tTA (left) or HoxA5-tTA mice (right) 4 days following administration of a 2.0 cm wound and staining with CD31. The upper panel shows lower magnification of positive CD31 staining in the dermis (d) adjacent to the proliferating epidermis (e). The middle panel shows higher magnification of the inset box for positive CD31 staining. The lower panels show corresponding staining with propidium iodide (PI). (C) Quantitative analysis of vascular density. Four days following wounding, HoxA5-tTA mice (n = 8) showed a significant reduction (p<0.05) in the number of CD31+ vessels compared to tTA mice (n = 4).